Effect of Preventive Acupunture Combined with CMNS6-1 Electroacupuncture Instrument on Promoting Apoptosis of 7,12-Dimethylbenzene (a) Anthracene-Induced Breast Cancer Model Rats

The purpose of this study was to evaluate the effect preventive acupuncture combined with CMNS6-1 electroacupuncture on 7,12-dimethylbenzanthracene induced mammary tumors in Sprague-Dawley rats and explore from perspective apoptosis. 48 w old female were randomly divided into control (10), model tamoxifen (10) groups. group, group administered once intragastrically 100 mg/kg body weight induce spontaneous breast cancer. On d 1 after modeling, plus electrical stimulation group. Density wave selected for a frequency 2 Hz/100 Hz an intensity mA three acupoints, "Zu San Li", "San Yin Jiao" "Tan Zhong", stimulated 20 min per treatment, every other day, times week 16 w. Tamoxifen (3 weight, intragastrically, day) started day administration continued w, as positive treatment. Apoptosis tumor tissue cells detected by terminal deoxynucleotidyl transferase dUTP nick-end labeling assay, expression caspase-3 B-cell lymphoma-2 assessed immunohistochemistry. During experiment, no control+sham all 7,12-dimethylbenzanthracene+sham developed cumulative incidence %, meanwhile 7,12-dimethylbenzanthracene+preventive 7,12-dimethylbenzanthracene+tamox 60 % 50 respectively. Terminal assay showed that could significantly promote apoptosis rat, while increased levels pro-apoptotic proteins such associated X. level antiapoptotic protein down-regulated ratio lymphoma-2/B-cell X increased. Preventive therapy instrument inhibited 7,12-dimethylbenzanthracene-induced tumorigenesis mechanism may be related regulation apoptosis-related promotion cell